Figure 1: Example peaks were chosen across the 1.5μl/min 30 min gradient. All peaks show excellent FWHM and symmetry through the entire gradient.

Figure 2: Asymmetry factors were calculated for all identified peptides, and plotted on a density plot. The Median asymmetry was equal the 1, indicating that a majority of peaks show a Gaussian distribution.

Reproducibility

CV by gradient and flow rate

Figure 3: HeLa tryptic digest was run on an Aurora Elite 15 cm x 150 μm column. Gradients 10min, 15min, 30min and 45min were run at flowrates 0.4 ul/min and 1.5 ul/min (4 replicates). Coefficient of variations of protein intensities were calculated across runs within each condition. Resulting calculations show sub 8% median CV across all samples.

Resolution

FWHM by gradient and flow rate

Figure 4: HeLa tryptic digest was run on an Aurora Elite 15 cm x 150 μm column at four different gradients and two flow rates. 10min, 15min, 30min and 45min, at both 0.4 and 1.5ul/min. Across all runs, the average FWHM is below 4 secs.