Aurora® Rapid™ 8×75 XT C18 UHPLC column
Aurora® Rapid™ 8×75 XT C18 UHPLC column
8 cm x 75 μm C18 UHPLC column with built-in HeatSync™ technology, compatible with EASY-Spray, Nanospray Flex and Newomics UniESI and DuoESI ion sources.
| SPD | 50 - 100 |
|---|---|
| Gradients | 10 - 30 mins |
| Recommended flow rate | 100 - 1000 nL/min |
Aurora® Rapid™ 8×75 XT C18 HeatSync™ Starter Pack
Maximal throughput for single-cell and low input samples
Generation 4 Aurora Rapid® 8×75 nano flow columns are purpose-built for high-throughput LC-MS workflows, delivering unmatched performance for the analysis of single cells and low-input proteomics. Designed with an ultra-narrow internal diameter and a compact 8 cm bed length, these columns enable exceptional sensitivity and separation efficiency, even in the shortest gradient times.
Whether you’re processing thousands of samples in a large-scale study or working with trace-level biological material, Aurora Rapid® 8×75 delivers unparalleled depth of proteome coverage without sacrificing data quality. The columns are optimised for ultra-fast gradients, reducing run times while maintaining precise peak shapes and reproducible retention times, crucial for confident identification and quantification in demanding experimental designs.
Ideal for laboratories prioritising speed, precision, and sensitivity, the Gen 4 Aurora Rapid® 8×75 columns set a new benchmark for throughput and performance in cutting-edge single-cell and low-sample-load proteomics.
Product Benefits
- High throughput
- Sensitivity
- Analyse more cells in less time
High reproducibility across multiple columns.
Unique protein group identifications remained highly consistent across four columns, with an average of 3,934 identified across all columns. Peptide identifications followed a similar trend, with an average of 25,308 unique
peptides – demonstrating robust performance.
Unique protein group identifications across different columns. A HeLa tryptic digest (250 pg) was separated on an Aurora Rapid 8 cm × 75 µm column using an 80 SPD method on a Vanquish Neo system coupled to a Bruker timsTOF Ultra 2 mass spectrometer. Three runs were performed per column. Raw data were analysed in Spectronaut version 19.8 with the “Match between runs” feature disabled.
Unique peptide identifications across different columns. A HeLa tryptic digest (250 pg) was separated on an Aurora Rapid 8 cm × 75 µm column using an 80 SPD method on a Vanquish Neo system coupled to a Bruker timsTOF Ultra 2 mass spectrometer. Three runs were performed per column. Raw data were analysed in Spectronaut version 19.8 with the “Match between runs” feature disabled.
Incredibly stable retention times
Retention times were highly consistent across multiple columns and injections, with minimal drift observed – this level of chromatographic reproducibility is critical for large-scale and longitudinal studies.
Stable peptide retention times across different columns. Ten peptides were selected and their retention times assessed across all columns from 3 HeLa tryptic digest injections (250 pg) on Aurora Rapid 8 cm × 75 µm columns, using an 80 SPD method. Samples were run on a Vanquish Neo and Bruker timsTOF Ultra 2 mass spectrometer. Raw data were analyzed in Spectronaut version 19.8 with the “Match between runs” feature disabled.
Column backpressure stability and protein group IDs over extended use
The figure below shows backpressure at 8 minutes over more than 650 QC runs using 60 SPD method, with only a gradual increase from 130 to 175 bar and consistently high unique protein group IDs. Complex samples were analysed between QC runs, highlighting column’s robustness and suitability for high-throughput proteomics.
Backpressure stability and protein group IDs over extended column use from HeLa tryptic digest QC injections (250 pg) on Aurora Rapid 8 cm × 75 µm columns, using a 60 SPD method. Samples were run on a Vanquish Neo and Bruker timsTOF Ultra 2 mass spectrometer. Raw data were analysed in Spectronaut version 19.8 with the “Match between runs” feature disabled.
| Column format | Analytical column |
|---|---|
| Column type | Reversed-phase |
| For use with | UHPLC |
| Length | 8 cm |
| Inner Diameter | 75 µm |
| Pore size | 120 Å |
| Pressure | >1700 bar |
| Temp. limits | 60°C |
| Particle size | 1.7 µm |
| pH stability | 1–8 |
| Stationary phase | C18 |
