Aurora® Rapid™ 5×75 CSI C18 UHPLC column
Aurora® Rapid™ 5×75 CSI C18 UHPLC column
5 cm x 75 μm C18 UHPLC packed emitter column, compatible with CaptiveSpray, CaptiveSpray 2 and CaptiveSpray Ultra ion sources.
| SPD | 80 - 300 |
|---|---|
| Gradients | 5 - 15 mins |
| Recommended flow rate | 100 - 2000 nL/min |
Maximal throughput for single-cell
and low-input samples.
Aurora Rapid® 5×75 delivers high-throughput analysis of single cells, achieving extreme sensitivity using short gradients. Identify more than 1300 proteins from 250 pg of sample, 80 times per day on an Evosep One and timsTOF SCP.
Product Benefits
- High throughput
- Nanoflow
- Sensitivity
- Analyse more cells in less time
Get more, from less.
Identify more than 1300 proteins from 250 pg of sample, 80 times per day on an Evosep One and timsTOF SCP.
A dilution series of a HeLa tryptic digest was separated on an Aurora Rapid® 5×75 column using an Evosep One and Bruker timsTOF SCP (dia-PASEF) using an 80 samples per day method (currently under development), n = 6. Data courtesy of Dr Christoph Krisp (Bruker), Dorte Bekker-Jensen and Dr Nicolai Bache (Evosep).
Lightning-fast peaks.
Maximum peak capacity and reproducibility for low sample inputs using short gradients.
A HeLa tryptic digest (1 ng) was separated on an Aurora Rapid® 5×75 column using a Thermo Scientific Vanquish Neo UHPLC and TSQ Quantis across a 15 min sample gradient at 100 nL/min. The extracted ion chromatograms for 7 selected peptides from the analysis are shown. The average Full Width at Half Maximum (FWHM, seconds) for each peptide across 30 runs is shown above each peak.
| Column format | Analytical column |
|---|---|
| Column type | Reversed-phase |
| For use with | UHPLC |
| Length | 5 cm |
| Inner Diameter | 75 µm |
| Pore size | 120 Å |
| Pressure | >1700 bar |
| Temp. limits | 60°C |
| Particle size | 1.7 µm |
| pH stability | 1–8 |
| Stationary phase | C18 |
