Aurora® Elite™ 15×75 CSI C18 UHPLC column
Aurora® Elite™ 15×75 CSI C18 UHPLC column
15 cm x 75 μm C18 UHPLC packed emitter column, compatible with all Bruker CaptiveSpray sources.
| SPD | 30 - 60 |
|---|---|
| Gradients | 10 - 45 mins |
| Recommended flow rate | 100 - 600 nL/min |
Our ‘experiment-in-a-day’ solution.
A product made for researchers, by researchers. The Aurora Elite™ is specifically tailored to achieve the deep discovery synonymous with Aurora Series™ columns, in cadence with researchers wishing to leverage ‘experiment in a day’ workflows.
Product Benefits:
- High throughput
- Exceptional coverage for rate of throughput
- Balanced throughput and depth of coverage
Spectacular protein IDs.
Every run. Every time.
More protein IDs. Less time.
Identify more than 6000 proteins per sample using our 50 samples per day method (20 min gradient).
Figures 1, 2 & 3: Identified unique proteins, peptides and average Full Width at Half Maximum (FWHM) from 80 ng HeLa Tryptic Digest injection on an Aurora Elite™ 15 cm x 75 μm column using different gradient lengths. Samples were run on a Waters ACQUITY UPLC M-Class coupled to a Bruker timsTOF Pro, dia-PASEF acquisition. Data analysed using DIA-NN.
Figure 4: Yeast tryptic digest was spiked into a HeLa tryptic digest (200 ng) in different ratios (Sample A – 45 ng; Sample B – 15 ng). Samples were run on a Waters ACQUITY UPLC M-Class coupled to a Bruker timsTOF Pro, dia-PASEF acquisition using an Aurora 15 cm x 75 μm column. Data analysed using DIA-NN. Each point represents a unique protein with ratios calculated between the A and B samples.
Figure 5: Boxplot demonstrating average ratio and interquartile range of data. Expected ratios are indicated with dashed lines.
High quantitative accuracy
Identify large numbers of unique proteins without compromising quantitative accuracy.
| Column format | Analytical column |
|---|---|
| Column type | Reversed-phase |
| For use with | UHPLC |
| Length | 15 cm |
| Inner Diameter | 75 µm |
| Pore size | 120 Å |
| Pressure | >1700 bar |
| Temp. limits | 60°C |
| Particle size | 1.7 µm |
| pH stability | 1–8 |
| Stationary phase | C18 |
