Generation 4 Aurora Rapid® 8×75 columns deliver high-throughput analysis of single cells and small sample loads, providing extreme sensitivity in shorter gradients. Achieve exceptional depth of coverage and high throughput without compromising data quality.

Product Benefits

• High throughput
• Nanoflow
• Sensitivity
• Analyse more cells in less time

Incredibly stable retention times

Retention times were highly consistent across multiple columns and injections, with minimal drift observed – this level of chromatographic reproducibility is critical for large-scale and longitudinal studies.

Stable peptide retention times across different columns. Ten peptides were selected and their retention times assessed across all columns from 3 HeLa tryptic digest injections (250 pg) on Aurora Rapid 8 cm × 75 µm columns, using an 80 SPD method. Samples were run on a Vanquish Neo and Bruker timsTOF Ultra 2 mass spectrometer. Raw data were analyzed in Spectronaut version 19.8 with the “Match between runs” feature disabled.

Column backpressure stability and protein group IDs over extended use

The figure below shows backpressure at 8 minutes over more than 650 QC runs using 60 SPD method, with only a gradual increase from 130 to 175 bar and consistently high unique protein group IDs. Complex samples were analysed between QC runs, highlighting column’s robustness and suitability for high-throughput proteomics.

Backpressure stability and protein group IDs over extended column use from HeLa tryptic digest QC injections (250 pg) on Aurora Rapid 8 cm × 75 µm columns, using a 60 SPD method. Samples were run on a Vanquish Neo and Bruker timsTOF Ultra 2 mass spectrometer. Raw data were analysed in Spectronaut version 19.8 with the “Match between runs” feature disabled.